The Electron Transport System of Hydrogenomonas eutropha

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The Electron Transport System of Hydrogenomonas eutropha

Wosilait and Nason (1) demonstrated that quinones could serve as electron acceptors for the enzymatic oxidation of reduced pyridine nucleotides, and their survey of plant, animal, and microorganism extracts (2) revealed the general occurrence of reduced pyridine nucleotide-quinone reductase activity. This finding and Dam’s (3) earlier report, which indicated that vitamin K was a common constitu...

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Mixotrophic growth of Hydrogenomonas eutropha.

Mixotrophic growth conditions were established by the addition of lactate to cultures of Hydrogenomonas eutropha growing autotrophically in a gaseous environment of H(2), O(2), and CO(2) (6:2:1). The specific growth rate of mixotrophic cultures was double that of the autotrophic cultures, and lactate disappearance paralleled growth. Growth yields in mixotrophic cultures were significantly great...

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Nutritional requirements for Hydrogenomonas eutropha.

Repaske, Roy (National Institute of Allergy and Infectious Diseases, Bethesda, Md.). Nutritional requirements for Hydrogenomonas eutropha. J. Bacteriol. 83: 418-422. 1962.-A simple apparatus for the autotrophic cultivation of Hydrogenomonas eutropha in 100-ml shake cultures is described. Nitrogen, in the form of ammonium, nitrate, or urea, was used for growth; nitrite could not be utilized. Opt...

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Yields of Hydrogenomonas eutropha from Growth

Molar growth yields were determined from chemostat cultures of Hydrogenomonas eutropha on succinate and on fumarate. The yields from culture on succinate were about 12 g higher than on fumarate. Assuming this difference to be equivalent to 1 molecule of adenosine triphosphate, it is concluded that the oxidation by oxygen of the Hydrogenomonas cytochrome b yields 1 molecule of adenosine triphosp...

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Factors affecting the synthesis and degradation of ribulose-1,5-diphosphate carboxylase in Hydrogenomonas facilis and Hydrogenomonas eutropha.

Hydrogenomonas facilis and H. eutropha cultured in fructose medium retained high levels of ribulose-1,5-diphosphate carboxylase only when the following conditions were fulfilled: low aeration, FeCl(3) addition to fructose medium, and cell harvest at or prior to mid-exponential phase of growth. Repression of carboxylase synthesis was demonstrated under conditions of high oxygen tension during gr...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1962

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)60333-3